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KMID : 0545119920020010001
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Journal of Microbiology and Biotechnology
1992 Volume.2 No. 1 p.1 ~ p.6
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Synthesis of α-L-Aspartyl-L-phenylalanine Methyl Ester from an Artificial Polypeptide
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Choi, Soon Yong
Kim, Hyun Soo/Lee, Se Yong/Bock, Robert M.
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Abstract
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The aspartame, α-L-aspartyl-L-phenylalanine methylester, is an artificial sweetener. Taking advantage of the fact that the aspartame is a derivative of dipeptide, synthesis of aspartame from the artificial polypeptide made by an artificial gene has been attempted. The artificial polypeptide (LAP32), a polymer of tripeptide (aspartyl-phenylalanyl-lysine), was purified from the E. coli cells harboring a recombinant plasmid containing the artificial gene. This polypeptide was then digested with trypsin and carboxypeptidase B to produce dipeptide (Asp-Phe). Using the esterase activity of α-chymotrypsin, the dipeptide was directly converted into Asp-Phe methyl ester in a water-methanol system. When the methanol concentration in reaction mixture was 25%, 50% of dipeptide was converted to the dipeptide methylester without producing any by-products.
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